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Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG),〔Verhoeff-Van Gieson (VVG) Staining Protocol for Elastic Fibers. IHC World. URL: (). Accessed on: January 2, 2013.〕 is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 1908.〔Puchtler H, Waldrop FS. On the mechanism of Verhoeff's elastica stain: a convenient stain for myelin sheaths. Histochemistry. 1979 Aug;62(3):233-47. IHC World. URL: (). Accessed on: January 2, 2013.〕〔Verhoeff's Stain. Merriam-Webster. URL: (). Accessed on: January 2, 2013.〕 The formulation is used to demonstrate normal or pathologic elastic fibers. Verhoeff's stain forms a variety of cationic, anionic and non-ionic bonds with elastin, the main constituent of elastic fiber tissue.〔 Elastin has a strong affinity for the iron-hematoxylin complex formed by the reagents in the stain and will hence retain dye longer than other tissue elements. This allows elastin to remain stained, while remaining tissue elements are decolorized. Sodium thiosulfate is used to remove excess iodine and a counterstain (most often Van Gieson's stain) is used to contrast the principal stain.〔Verhoeff's Elastic Stain. TV Mouse UC Davis. URL: (). Accessed on: January 2, 2013.〕 Elastic fibers and cell nuclei are stained black, collagen fibers are stained red, and other tissue elements including cytoplasm are stained yellow. ==Reagents== Verhoeff's stain is a combination of the following reagents:〔 * Haematoxylin * Iron(III) chloride * Lugol's iodine * Van Gieson's stain * Acid fuchsin * Picric acid * Sodium thiosulfate 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Verhoeff’s stain」の詳細全文を読む スポンサード リンク
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